Role of class 1 Integron in prevalence extended-spectrum beta-lactamases of class D (oxa-2 , oxa-10) along with determining antibiotic resistance pattern in Pseudomonas strains isolated from clinical specimens

Aim and Background:Eradicating infections is difficult due to the presence of genes on the introns, especially the beta-lactamase enzymes and  The importance of their resistance is high.The purpose of this study was to determine the beta-lactamase genes (OXA-2 , OXA-10) , Integron class 1  and antibiotic resistance. Material and methods:In this cross sectional descriptive study, 120 Pseudomonas isolates were collected from Shiraz Burn Hospital during summer of 1395. After confirmation by biochemical tests, The presence of beta-lactamase genes was evaluated by using antibiotic discs and PCR method. Results :78.5% of the isolates had OXA-10 gene, 77.57% of the OXA-2 gene, 34.58% containing Int1, and 95.38% of the samples were MDR.Maximum resistant was seen in cefepime(79.43%) and the minimum was for to tobramycin(10.28%). Conclusion :Class1 integron, 2-oxa and 10-oxa beta-lactamase genes, did not show up a meaningful relationship at the same time.the production of beta-lactamase enhances the increasing resistance and their frequency is increasing according to the results of other studies. This will lead more control over the prescription and use of antibiotics.